![]() ![]() BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. After incubation, Tag-it Violet™ labeled cells are ready for downstream applications or analysis. Pellet cells and resuspend in pre-warmed cell culture medium.ĩ. Quench the staining by adding five times the original staining volume of cell culture medium containing 10% FBS.ħ. Incubate cells for 20 minutes at room temperature or at 37☌, and keep protected from light.Ħ. Spin down and resuspend cells at 10-100 x 10 6 cells/mL in the Tag-it Violet™ working solution.ĥ. Prepare a 5 µM working solution by diluting 1 µL of 5 mM Tag-it Violet™ stock solution in 1 mL PBS for every 1 mL of cell suspension (or at an optimal working concentration as determined by titration).Ĥ. Prepare stock solution by reconstituting 1 vial of lyophilized Tag-it Violet™ dye in 50 µl of anhydrous DMSO to make a 5 mM solution.ģ. Each 122.25 µg of Tag-it Violet™ Proliferation and Cell Tracking Dye may be reconstituted with 50 µl of anhydrous DMSO to yield a stock concentration of 5 mM.ġ. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.Ģ. The maximum excitation and emission wavelengths of Tag-it Violet™ Proliferation and Cell Tracking Dye are 395 nm and 455 nm, respectively. The molecular weight of Tag-it Violet™ Proliferation and Cell Tracking Dye is 489 kD. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application. It can be used at concentrations ranging from 1 - 20 µM for cell labeling. This lot has been tested by flow cytometry for in vitro cell proliferation. Once the DMSO is added to the Tag-it Violet™, use immediately, or store at -20☌ in a dry place and protected ifrom light, preferably in a dessicator or in a container with dessicant for no more than one month. ![]() ![]() The Tag-it Violet™ Proliferation and Cell Tracking Dye should be stored at -20☌ upon receipt. For reconstitution, bring the kit to room temperature, add 50 µl of DMSO to one vial of Tag-it Violet™ dye until fully dissolved. The Tag-it Violet™ Proliferation and Cell Tracking Dye is composed of lyophilized Tag-it Violet™ and anhydrous DMSO. ![]()
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